IUBio

Immunoprecipitation with IgM

immunechem immune at intergate.bc.ca
Thu Apr 8 14:33:00 EST 1999



Yes! But you can also use protein A/G using rabbit anti-mouse IgM as 
bridge

>Does anyone have a protocol for immmunoprecipitations with IgM 
monoclonal Abs.
>Specifically what do you use to pull down the antigen-antibody complex
>with? Can I use biotinylated anti-mouse polyvalent Igs (Sigma Cat. No.
>A9207) and Avidin-agarose (Sigma Cat. No. B2016)?
>
>Any help greatly appreciated,
>
>Ian Mc   
>
>Confused? I am.




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