In article <37195CEB.76FD79FE at mail.med.upenn.edu>,
Behjatolah Karbassi <karbassi at mail.med.upenn.edu> wrote:
>I want to block the MHC class II function in vitro. I use spleenocytes
>from Balb/c mice and anti IA-d antibody from Pharrmingen, is it going
>to block the function completely or any reccommendation?
>Thank you.
This will probably be fine as long as you only care about I-Ad, but Balb/c
also express I-Ed.
An alternative (and far less expensive) approach is to use cholorquine,
which prevents endosomal acidification and therefore blocks antigen
processing at a relatively early point.
Ken Frauwirth
--
Ken Frauwirth (MiSTie #33025) kfrauwir at midway.uchicago.edu
Gwen Knapp Center for Lupus and Immunology Research
University of Chicago
http://www.ocf.berkeley.edu/~frauwirt
