Esterase activity is preserved by fixation in 2 or 4% paraformaldehyde.
Gordon
"Dirk Jochmans, HQ Mechelen, Belgium" wrote:
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>> We would like to perform a non-specific esterase stain using alpha-naphtyl =
> acetate. We would like to do this in a plastic culture bottle in which =
> adherent macrophages/monocytes are cultured. The problem is the fixation =
> step. A lot of kits use aceton in their procedures, we believe this could =
> react with the plastic.=20
> Is there somebody out there that already did a non-specific esterase =
> staining in culture bottles, and what fixation procedures did you use?
> Or is there a way to collect the monocytes,/macrophages without loosing =
> their esterase positivity?=20
>> Thanks,
> Dirk Jochmans
>dirk.jochmans at tibotec.be=
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