IUBio

ELISA protocol

TGAco tgaco at aol.com
Sat Apr 24 08:31:56 EST 1999


Sarah-
I don't know wht type of assay you are planning on running, but usually, if you
coat overnight at 4degrees in 1xPBS or Borate at a concentration of 2ug/ml,
depending upon what type of assay it is, that will be sufficient.  Also, don't
forget to block with 1%bsa or milk before you add the supernatants or samples
to eliminate any non-specific signal.  Good luck and please don't hesitate to
e-mail or call should you have any questions.

Michael Settles
TGA Sciences, Inc.
TGAco at aol.com
978-927-1085



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