TUNEL reaction can not label apoptosis very specifically, apoptosis and necrosis
can present with DNA nicks --> thus labelled by TUNEL method
wtc
"D. De Groote" ¼g¹D¡G
> *Does the TUNEL reaction really differentiates apoptosis and necrosis
>> *After phagocytosis of facultative intracellular bacteria by macrophages,
> some bacteria get killed by the marcophages, some can survive intracellular.
> On the other hand some macrophages survive, some undergo apoptosis, some
> necrose.
>> Can the techniques used to demonstrate apoptosis make a distinction between
> 'apoptotic' cells and killed intracellular bacteria ?
> Which technique would you recommend to make this distinction ?
>> Thanks
> Isabel
