ELISA protocol

mark mark.haynes at mail.tju.edu
Tue Apr 27 17:47:23 EST 1999


julia wrote:
> 
> Hi everyone,
> 
>     I'm planning on doing an ELISA next week, but I don't remember (and
> can't find) the protocol that I used before.  If you have any tips on
> how to coat a plate and do the assay, I'd really appreciate it!
> 
> Thanks in advance,
> -Sarah


Lab-books often help.
that said, i've coated plates with proteins and membranes suspended in a 
carbonate buffer ph 9.? (its all way in the past). You might try calling 
companies that made the plates you intend to use, though. good luck
markh



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