neutrophil isolation

Dr. Rachel Teitelbaum teitelba at
Thu Feb 4 16:21:02 EST 1999

I found that when I used two different density gradients of Ficoll (sigma,
histopaque), and layered the blood on top, I got much cleaner separation.
I often did not even need to lyse RBCs.

Dr. Rachel Teitelbaum
Department of Microbiology and Immunolgy
Albert Einstein College of Medicine

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