I am isolating human PBMC using 'Lymphoprep'. Some times it works but
nearly 50% of separations result in PBMC clumping together and either
sinking to sit on the RBC or forming a 'membrane' at the interface of the
ficoll/buffer. I have tried using PBS and RPMI, I have heard a citrate
buffer may prevent this - does anyone have a recipe? Or any other advice
or explaination why this happens?
Thanks
