Antigenicity of polypeptide

duheng duheng at netchina.com.cn
Sun Feb 14 08:42:33 EST 1999


Hello,
I plan to gain antibodies by immuning animals with a polypeptide whose
gene sequence has been known. I am thinking of expressing only a part of
this polypeptide possessing antigenicity as antigen. It is reported that
the last 20 amino acids of other peptide has been used. I would be
grateful if you would like to tell me whether this final portion of the
polypeptide is hydrophilic and easily accessed by antibodies. Can I
express the corresponding genes of my protein as antigen? Or which part
else of the protein is better?

with best regards
Chang Qin
Biotechnical Institute of Medicine
Peking Union Medical College
qchang at hyper.netchina.com.cn



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