B cell purification from spleen

mark mark.haynes at mail.tju.edu
Wed Feb 17 20:03:09 EST 1999

Chen, Peter wrote:
> Our lab is trying to find an efficient method to purify B cell populations
> from the spleen using magnetic beads or panning techniques. Is there an easy
> way to get rid of the myeloid cell populations? (we have found that
> macrophages make up only a small percentage of this cell population and
> using Ab to select them out would not increase the B cell purity
> significantly). Thanks.

Magnetics would be a good choice.  I'm sure papers by kelsloe or kelso or 
something, or time mansers group would have some techinical avenues for 
you to read about

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