Michael J. Richards wrote:
>> More than likely what you had was plasma as opposed to serum since you were
> using anticoagulated whole blood. That being the case and depending on the type
> of anticoagulant you used the plasma could be made to clot by adding excess
> calcium which may have come from your MEM media
>> Andrew Hall wrote:
>> > I came across an unusual phenomenon today whilst separating human PBMC from
> > whole blood over Ficoll Hypaque. After removing the buffy layer and spinning
> > it down the supernatant appeared to solidify. I was unable to recover any
> > cells from this prep. The supernatant contained serum and MEM alpha medium.
> > The serum from the sample (taken separately) was very cloudy. Does anyone
> > have any suggestions as to why this should happen.
>> --
> Michael J. Richards
> Research Biochemist
Correct Sir!
