In article <76f10c$o4$1 at nnrp1.dejanews.com>, yangh at mayo.edu wrote:
> Does anyone has the experience to succesfully stain the monocytes for FACS?
> because the monocyte has a lot of Fc Receptors which will nonspecificlly bind
> the conjugated antibodies even with the control IgG(Simultest). any comments
> will be really appreciated.
Try 'staining' your monocytes in FACS buffer with an overdose of unlabeled
irrelevant antibodies first, then add the (labeled) relevant staining (do
not wash in between), then wash as usual. The irrelevant antibodies of the
same isotype as your staining mAbs will block all avialable binding sites
prior to and during staining, so background is greatly reduced.
Guy
--------Guy Hermans, PhD student------------
MS research Unit Tel 0032 (0) 11/26.92.07
Dr. L. Willems-Institute Fax 0032 (0) 11/26.92.09
Belgium
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