IUBio

Calculation of antibody concentration

Joe Chandler mbiotec at maine.rr.com
Thu Jan 14 10:01:03 EST 1999


I think you can use 1.36 as the extinction coefficient.  I did not think
the species from which the anibody was derived matter.  I will be currious
to hear other opinons on this.

"Gelzleichter, Thomas R" <gelzleit at BATTELLE.ORG> wrote in article
<1BC05949CA44D11187CD00A0C9845579BA7DCB at ns-bco-mse6.im.battelle.org>...
> I am in search of an extinction coefficient for horse IgG that would
allow
> me to calculate protein concentration of horse IgG at Absorbance 280 nm. 
Is
> there a reference source available where I could find this info?
> 
> Thanks,
> 



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