Guy Hermans wrote:
>> In article <369DC17F.F600EFAE at hotmail.com>, Cattedra di Immunologia
> <immunologia at hotmail.com> wrote:
>> > We work in an Immunobiology lab in Milan, and we're trying to make up
> > some ELISpot protocols. We are finding some difficulties in obtaining
> > spots on plastic plates and in evaluating spots... Can you tell us
> > something about these problems ?
>> We're using the nitrocellulose-bottom Millipore plates, and have had
> perfect results with those from the very start. Because we read some
> articles on plastic plates, we decided to try a number of ELISA plates -
> they're A LOT cheaper, and some claim to have even better results with
> plastic plates. Unfortunately, despite changes in ELISA plates, buffers,
> protocols etc., we still have to see our very first spot.
> In short: nitrocellulose is expensive, but it works fine. Plastic is
> cheap, but doesn't work at all, despite what others have published.
>> Hope this resolves some of your self-doubts, probably caused by the very
> large and frustrating difference in publications and practical results,
> just as we experienced. Cheers,
>> Guy
>> --------Guy Hermans, PhD student------------
> MS research Unit Tel 0032 (0) 11/26.92.07
> Dr. L. Willems-Institute Fax 0032 (0) 11/26.92.09
> Belgium
> --------------------------------------------
Im just gettin started regarding the elisapot assays. Is there a reference
that you could post where they explain the technique well?
I know the idea but not the pecularities, if there are any, regarding the
culture conditions and etc.
thanx
markH
