In article <369DC17F.F600EFAE at hotmail.com>, Cattedra di Immunologia
<immunologia at hotmail.com> wrote:
> We work in an Immunobiology lab in Milan, and we're trying to make up
> some ELISpot protocols. We are finding some difficulties in obtaining
> spots on plastic plates and in evaluating spots... Can you tell us
> something about these problems ?
We're using the nitrocellulose-bottom Millipore plates, and have had
perfect results with those from the very start. Because we read some
articles on plastic plates, we decided to try a number of ELISA plates -
they're A LOT cheaper, and some claim to have even better results with
plastic plates. Unfortunately, despite changes in ELISA plates, buffers,
protocols etc., we still have to see our very first spot.
In short: nitrocellulose is expensive, but it works fine. Plastic is
cheap, but doesn't work at all, despite what others have published.
Hope this resolves some of your self-doubts, probably caused by the very
large and frustrating difference in publications and practical results,
just as we experienced. Cheers,
Guy
--------Guy Hermans, PhD student------------
MS research Unit Tel 0032 (0) 11/26.92.07
Dr. L. Willems-Institute Fax 0032 (0) 11/26.92.09
Belgium
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