IUBio

Horseradish peroxidase inactivation my metals

Emmanuel nony at club-internet.fr
Fri Jan 22 02:18:57 EST 1999


On 21 Jan 1999 02:49:10 GMT, "Graeme" <gbarney at ozemail.com.au> wrote:

>It is commononly recognised that horseradish peroxidase is inactivated by
>metals and that HRP conjgated antibodies etc must be kept away from
>potentential metal contamination.
>
>Does anyone know the chemistry involved in such inactivation or suggest any
>references about this please?

I am afraid I do not know much about this issue...however, even NaN3 will
dramatically reduce HRP activity ...
I am also interested in this inactivation process.

Emmanuel.
nony at club-internet.fr



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