Paula Lavery wrote:
>> I would like to immunize mice with plasmid DNA in order to get monoclonal
> antibody. In order to get the best response of I have to injure the muscle
> with cardiotoxine 5 days prior to DNA injection. I do not have the great
> success with cardiotoxine injection. Death rate of mice is more than 50%.
> The concentration of the cariotoxine is given to mice 100 micromolar in
> PBS, 100 microliter bilateral injection. If you can suggest some advice, it
> would be helpful.
>> thankyou!
>> Archana
> Transplant Immunology Lab.
> Royal Victoria Hospital
> McGill University
McGill has some pretty sharpe experimental immunologists from what i
remember (murgita, baines,lapp) they sohould be able to answer you.
good luck
