Hello,
I am planning to assay chinook salmon plasma for lysozyme concentration
using the agarose plate technique. Would anyone be able to clarify for me
the following:
1. What pH should the sodium phosphate buffer be? (I have seen in the
literature a range of 5.8 to 6.24)
2. What is the wavelength at which the turbimetric assay (to determine
lysozyme activity) should be performed (I have seen both 530 and 450
referenced)
Thanks for your help,
C. Bryden
M.Sc. Candidate (Biology)
College of Science and Management
University of Northern British Columbia
Prince George, B.C.
