On Wed, 28 Jul 1999, James F. George, Ph.D. wrote:
> We have found that cells stored in this manner tend to degrade the RNA after
> a few weeks to months. We have found it to be more stable if we lyse them
> in Tri-reagent (a commercial variant of the acid-phenol extraction lysis
> buffer) and then store them at -70C.
I ahev contrasting experiences ! In my hands, freezing tissue or blood
rapidly in liquid N2, and then storing at -70 is much better than storing
in TriZol at -70.
Kind regards,
Clemens Suter-Crazzolara, PhD
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