PBMCs

kay kimxtyus at wpsmtp.siumed.edu
Wed Jun 23 07:55:55 EST 1999


I am trying to sort out naive T cells (CD45RA+). This is a subset of
PBMC,of CD3+T cells of CD3+CD4+ T cells.  Last time, I did not get
enough cells from 17ml of blood for my stimulation experiment ( I need
at least 2,500,000.  I need to know the number of lymphocytes in say a
ml of blood and the percentage of each subset I should expect out of
that number.  That way I can save myself some BLOOD, sweat and tears.



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