Nanci Donacki nancieed at earthlink.net
Fri Jun 25 20:43:47 EST 1999

The average recovery is 1-2 million/ml of blood. for PBMC.  If you separate
out subsets, you will need to start with much more.
kay <kimxtyus at wpsmtp.siumed.edu> wrote in message
news:3770D95B.7CC8 at wpsmtp.siumed.edu...
> I am trying to sort out naive T cells (CD45RA+). This is a subset of
> PBMC,of CD3+T cells of CD3+CD4+ T cells.  Last time, I did not get
> enough cells from 17ml of blood for my stimulation experiment ( I need
> at least 2,500,000.  I need to know the number of lymphocytes in say a
> ml of blood and the percentage of each subset I should expect out of
> that number.  That way I can save myself some BLOOD, sweat and tears.

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