Cloning with FACS sorter

Chidi ngwu at helix.mgh.harvard.edu
Tue Mar 2 10:15:38 EST 1999


Hi,

Has anyone tried subcloning hybridomas with a FACS sorter? I want to get
away from manual single cell picks and serial dilutions. It will be nice to
throw in recently fused cells (B cells to Myeloma fusions) and sort them
into singles.
--
Chidi

-- Do unto others as you will have them do unto you --



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