Cloning with FACS sorter

Chidi ngwu at helix.mgh.harvard.edu
Thu Mar 4 10:20:40 EST 1999


Terry,

Thanks for your help. It is refreshing to know I am twenty years behind, I
only wish I was twenty years younger too! Is it possible for you to email
the web page to me (I have a html mail reader)? The web page seems to be
sitting on a restricted server.

I really wonder if the sorters sort the cells sterilely, and if you have to
label the cells with a fluorescent dye? What is the viability and
conditions?

You are right, I only want to sort at about day six, after fusion and
plating in selection media.

--
Chidi





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