Transfecting B-lymphocytes

[Tracy McGaha (Microbiology)]

Boehringer Mannheim has a product called Fugene 6 which works very well
with primary cell cultures. For a protocol try current protocols in cell
or molecular biology. Good luck!

On Mon, 1 Mar 1999, Ian Mc wrote:

> Hi,
> 
> Does anyone have a protocol for transiently transfecting B-lymphocytes
> from human blood ? Or could you suggest a reference or a product which I
> could use.
> I tried searching medline but only got a thousand and one articles on
> b-cell lines.
> 
> Thanks
> 
> Ian Mc
> 
>