PBMC & conA, help!

Jinguo Wang n84jw at morgan.ucs.mun.ca
Wed Mar 10 19:59:32 EST 1999




Hi, everyone!

I met some problem with my conA stimulated PBMC. There are always big
clumps formed and the cells inside died without enough nutrition. I try
to separate the clumps or decrease the conA concentration to 10ug/ml,
but I failed. I need to do some RNA extration by TRIZOL and it is very
difficult for me to get enough RNA for RT-PCR. So, could you give me any
suggestions? 

Thank you in advance for any kind of help!

J. W.
n84jw at morgan.ucs.mun.ca
MUN








More information about the Immuno mailing list