siliconised glassware

Carol Iversen carol at
Sat Mar 20 13:55:10 EST 1999

Why is it necessary to siliconise glassware used in PBMC isolation and what
is a good protocol.  I have heard of the following:
Immerse glassware in 1% silicon solution for 10 secs
Rinse x 10 in distilled water
Is fumed or amorphous silica used?
Thanks for any help

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