siliconised glassware

Carol Iversen carol at try-again.freeserve.co.uk
Sat Mar 20 13:55:10 EST 1999


Why is it necessary to siliconise glassware used in PBMC isolation and what
is a good protocol.  I have heard of the following:
Immerse glassware in 1% silicon solution for 10 secs
Rinse x 10 in distilled water
Autoclave
Is fumed or amorphous silica used?
Thanks for any help





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