Replacement of "old" 3H-thymidine assay.

Jens Rainer Hansen jens.rainer at leo.dk
Wed Mar 24 10:10:10 EST 1999


I want to replace my 3H-thymidine assay on human lymphocytes, since it is
to hard to perform when having a lot of samples.

I have tryed alamarblue but it doesnt work with lymphocytes.
The MTT assay should also be insensitive with these cells.

I have also tryed Brdu incorporation it seems ok, some times, but since the
lymphocytes are non adherent it is nessesary to dry the cells on the
microtiterplates with an hairdryer, and this procedure was not stable for
me.

Do you have experience with an easy assay to meassure DNA synthesis/cell
proliferation on human lymphocytes (non adherent) using other methods than
3H-thymidine, or methods where it is easy to handle the samples using
3H-thyminine, that means not punching filters into vials etc.


Yours Jens R. hansen



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