how can I cultire m,onocytes?

[Jay and Nancy Mone]

If you're talking about peripheral blood mononuclear cells, trhe simplest 
way  is to separate the mononuclear fraction using centrifugation through 
Histpopaque.  The mononuclear cells can be harvested and cultured at 1 x 
10(6) cells/ml in MEM with 10% FBS, glutamine, and non-essential amino 
acids.  Monocyte/macrophages will adhere to the surface of the flask, and 
the lymphocytes won't.  Besides, the lymphocytes will die in a few days 
without any IL-2.  You can confirm the identity of the cells using a 
non-specific esterase stain, which will be positive for macrophages.

Jay Mone'