MRC-5(ATCC CCL171) cell culture

kobitech kobitech at chollian.net
Sat Mar 27 12:44:13 EST 1999


Dear Sir!
I have another question to you.
When I culture MRC-5(CCL-171) in 60mm dish and 100mm dish, we can get
satisfied cell number,
2,000,000 cells/60mm dish with 3ml DMEM(low glucose,10% FBS) and 6
~7,000,000 cells/100 dish with10ml DMEM(low glucose,10% FBS)
But I can not get enough cell number in 150mm dish and 490cm2 roller
vessel,
8,000,000 cells/150mm dish with 15~20ml DMEM(low glucose,10% FBS)
and 20,000,000 cells/490cm2 roller vessel with 50~100 ml DMEM(low
glucose,10% FBS).I have done these test at passage number 26 and 27.
I want  get much more cell number same area in 490cm2, at least
40,000,000
when I plating 1~1,500,000cells. How to overcome this problem.
Could you please inform to me about that or introduce a expert of MRC-5
culture.
Thank you .

With best regard.





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