Lymphocyte proliferation assay with specific antigen stimulation
luck_felder at hotmail.com
Tue Mar 30 09:18:38 EST 1999
Hello Hua-Chen Chang!
Hua-Chen Chang wrote:
> However, I have heard that live virus can also do the work. The reason for
> inactivated virus for stimulation in lymphocyte proliferation is to provide a
> MHC class II processing pathway or avoid the cell killing by the virus, or any
> particular reason?
You have to avoid cell killing, if You want to compare Your results
between different cell-probes with discret cell-numbers.
> Does MHC class II processing pathway is still required for in vitro lymphocyte
> proliferation assay? CTL assay by standard Cr-51 release assayt does not need
> a live virus for stimulation.
For antigen processing and presentation via MHC protein You don´t need
any living antigen. You need epitopes which can be recognized by Your
> Is APC required for nonspecific stimulation with ConA in this assay?
If ConA works as a mitogen, as it should, You don´t need APC´s, what
also means that You don´t need MHC II processing for a proliferation
> It only takes two days for ConA stimulation. How many days does this assay
> need for antigen specific stimulation?
If You have a running system, You should always compare Your result out
of a new system to the results of Your running system. In my opinion,
You should start with 48h, which is a common time for proliferation
> Thank you all.
> Hua-Chen Chang
Hope, helped You.
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