I am having a problem using candida as a stimulator for peripheral blood lymphocytes

Christina Kaufman clkauf01 at GWISE.LOUISVILLE.EDU
Thu May 6 08:06:44 EST 1999


I am having a problem using candida as a stimulator for peripheral blood
lymphocytes 
(human) in thymidine uptake proliferation assays.  Using CFDASE I noticed
that B cells 
were also proliferating, and it turns out that my commercial source (Greer)
does not test 
for endotoxin, and in fact my candida has a good bit of LPS in it.

Is anyone else using candida for in vitro proliferation assays, and if so,
how are you 
dealing with the endotoxin contamination?



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