I am having a problem using candida as a stimulator for
peripheral blood lymphocytes
clkauf01 at GWISE.LOUISVILLE.EDU
Thu May 6 08:06:44 EST 1999
I am having a problem using candida as a stimulator for peripheral blood
(human) in thymidine uptake proliferation assays. Using CFDASE I noticed
that B cells
were also proliferating, and it turns out that my commercial source (Greer)
does not test
for endotoxin, and in fact my candida has a good bit of LPS in it.
Is anyone else using candida for in vitro proliferation assays, and if so,
how are you
dealing with the endotoxin contamination?
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