Can Coomassie Blue-stained protein be used for ELISA and spleenocyte proliferation assays?

Santosh N. Pawar santi at MCBL.IISC.ERNET.IN
Sat May 8 06:39:30 EST 1999

Hi Everybody,

 I have purified a protein by electroeluting from 10%SDS-PAGE gel after
coomassie-blue staining. So the final protein solution which i have is
blue in colour due to the coomassie stain complexed to the protein. Can
i use this protein solution directly for injecting into mice to see
immune response to my protein? Also can i use this directly for
spleenocyte proliferation studies? Or do i have to get rid of this
coomassie stain somehow and then use the protein? If anybody has any
idea regarding this, please let me know. It would be highly appreciated.
 Thanking you all
 Santosh Pawar

email - santi at mcbl.iisc.ernet.in

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