Complement and Sheep Red Cells

F. Hayashi hayashi at u.washington.edu
Mon May 17 23:00:34 EST 1999

On Fri, 14 May 1999, Derek W.R. Gray wrote:

> Has anyone any information on the difference between human plasma and
> serum in terms of activation of the alternative pathway of
> complement-mediated lysis of sheep red cells?  We are using this has a
> positive control for another assay of the effect of complement (on
> xeno-genetic islets), and have found that plasma has a remarkable
> inability to lyse sheep red cells.  Is this on the basis of complement
> inhibitors ?  I would suspect this is the sort of information that was
> worked out many years back, I will be grateful if anyone can search
> their memory for information or source of expertise.

How are the serum and plasma prepared?

| Fumitaka Hayashi      -       hayashi at u.washington.edu      |
| http://macrophage.immunol.washington.edu/~fumi/index.html   |
| Aderem Lab - Dept. of Immunology - University of Washington |

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