Andrew awalley at radius.jr2.ox.ac.uk
Wed May 26 10:27:28 EST 1999

anlong xu wrote:

> The nucleotide sequence were performed to verify one point mutation
> between two PCR products, but our PCR-SSCP can not detect the
> differentiation. I will be grateful if anyone can tell me why.

SSCP detects differences in conformation, so if your point mutation does
not cause a significant change in conformation then it will be
undetectable by SSCP.

Dr  Andrew Walley

Asthma Genetics Group,
Wellcome TRust Centre For Human Genetics,
Oxford, UK

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