a-factor sequence cleavage

Bin Tang btang at POST.ITS.MCW.EDU
Wed Nov 24 16:55:55 EST 1999


Hi, Dear Sir/Madam:

What I am doing now is cloning and expressing Asp f 2 gene with EasySelect
Pichia Expression Kit. I would like to cut the a-factor signal sequence
after I get the recombiant protein. So, any information about Kex2 signal
cleavage or Ste13 signal cleavage will be very appreciated. 

Happy Thanksgiven Day!

With my best wishes to you!

Your sincerely,

================================================================================
Dr. Bin Tang
Research  Service 151-I
VA Medical Center
Medical College of Wisconsin
5000 West National Avenue
Milwaukee, Wisconsin 53295
USA
Phone:414-384-2000ext1517(O)
      414-774-5095(H)
Fax:  414-382-5374
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