25 D-1 antibody

[Eran Nadav]

Dear Kank Liu,

We perform FACS with 25D1 Ab as a routine. You can read in our last
paper
Ben-Shahar S., J Biol Chem 1999;274(31):21963-72
or, go to the original article of Porgador A (from Ben-Gurion
University, Israel)
Porgador A. Immunity 1997 Jun;6(6):715-26
We never used fixed cells.

Good Luck,

Eran Nadav
Tel-Aviv University, ISRAEL

Kang Liu wrote:

> Anyone has the experience with the antibody 25D-1 which recognizes
> SIINFEKL
> on Kb molecules? Does it give good staining signal in FACs? How about
> the
> staining of the cells fixed with acetone?
>
> Kang
> *********
> Kang Liu
> The Rockefeller University
> 1230 York Ave. #114
> New York, NY 10021
> tel: 212-327-8114
> fax: 212-327-8875
> liuk at rockvax.rockefeller.edu