Expressing my protein in B cells

Fehr fehr at biozentrum.uni-wuerzburg.de
Tue Oct 19 13:28:57 EST 1999


Usually immunogobulin promoters give a very strong expression in B and
plasmacytoma cell lines, both human and mouse. An additional Ig-enhancer
might rise the expression levels a bit further. There have been some articles
by Karriolainen et al. (Basel Institute of Immunology) on that topic.
In addition, for human cell lines you may use EBV-based vectors like the pREP
from Invitrogen, which are replicating as episomes, resulting in relatively
high copy numbers.

I hope this may help you a bit.

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