FK506 (tacrolimus) in vitro

Paula Lavery plaver at
Tue Oct 26 14:35:17 EST 1999

We are having problems getting FK506 into solution to use in some in vitro
assays. It readily dissolves in EtOH (0.2M) but it precipitates out whenever
we try to dilute it further into culture medium. We tried adding some Tween
80 to the solution but it didn't help. Anyone have any bright ideas?

Paula Lavery
Transplant Immunology Lab.
Royal Victoria Hospital
McGill University
Montreal (QC) Canada

"If we knew what we were doing it wouldn't be called research, would it?"
-- Albert Einstein

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