Protein+alum incubation overnight?

C Coward cc122 at mole.bio.cam.ac.uk
Thu Sep 2 07:23:50 EST 1999


In a planned experiment I am intending to incubate a test protein
overnight at room temperature in the presence of alum prior to
innoculation. I know the protein is stable in PBS at room temperature
overnight and can generate an immune response. Question: is incubation
with alum likely to cause damage to the protein that may affect its
immunogenicity?

Cheers in advance for any replies.




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