Sonication of Vac prior to infecting CTL targets?

Geoffrey Cole gcole at PANGAEA-PHARM.COM
Wed Sep 8 11:07:47 EST 1999


I'm seeking informed input re: sonication of vaccinia virus prior to its
use for infecting CTL targets.
Does failure to sonicate fresh thawed aliquots of virus (infected cell
lysates) significantly reduce available infectious virus?  Is placement
of a thawed vial of virus in an open bath sonicator for 1-2 minutes
sufficient to break up virus aggregates?  In my current lab I've
encountered some debate over the necessity for sonication or whether a
probe type device is preferable to a bath.  No one here, however, has a
real empirical familiarity with this issue.

Any comments would be greatly appreciated.   Thanks in advance.

Geff Cole




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