MTT test for T cell proliferation?

Kenneth Frauwirth kfrauwir at midway.uchicago.edu
Wed Sep 8 13:03:21 EST 1999


In article <37D624D8.F1EB7990 at mont.nbu.acad.bg>,
Sashka Angelova Hristoskova <christos at MONT.NBU.ACAD.BG> wrote:
>Dear netters,
>
>Has anyone done MTT test instead of 3H thymidine incorporation for
>measuring of antigen specific T cell proliferation in PBMC culture? Any
>tricks of the trade.

As far as I know, MTT cannot be used to measure proliferation per se.  It
measures viability (the conversion reaction requires functional 
mitochondria, but does not require cell division).  The MTT assay can
replace 3H-thymidine uptake for IL-2 measurement by CTLL cells, since IL-2
promotes both survival and proliferation in CTLL, but it isn't terribly
useful if there may be significant numbers of viable, but 
non-proliferating, cells.

Ken Frauwirth
-- 
Ken Frauwirth (MiSTie #33025)  kfrauwir at midway.uchicago.edu
Abramson Cancer Research Institute
University of Pennsylvania
http://mail.med.upenn.edu/~kfrauwir



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