Immunogenic peptides' sequences - URGENT!!!

karlster karlster at excite.com
Fri Sep 10 08:37:13 EST 1999


[posted and mailed]
Hello Cattedra:

Some of the peptide names you listed are going to be pretty hard to locate
if there is no supplementary information (eg. author, journal, year).
Reason - most search engines like PubMed only search title, text and
keywords. 

> NS03
> HS41
> P17
> NS42
> C07.

Only P17 peptide for HCV I could find is:

Yao Hsueh Hsueh Pao 1996;31(10):751-6

[Studies on synthetic peptide. XX: the antigenicity and linear epitope map
of synthetic peptide hepatitis C virus].

[Article in Chinese]

Liu G, Liang ZL, Cai MS, Zhuang H, Guo JP, Tao QM

Hepatitis C virus (HCV), the major causative agent of post transfusion
non-A, non-B hepatitis (NANB), had been cloned and expressed. According to
the protein sequence of HCV-BK and its epitope profiles which combined the
hydrophilicity, accessibility, flexibility, antigenicity, charge
distribution and HPLC reserve coefficient of protein using the "Goldkey"
computer program, we designed and synthesized the following peptides:
P1(475-495), P3(449-468), P4(658-663), P5(645-663), P6(484-489),
P7(475-489), P15(655-662), P16(230-237), P17(225-237), P18(1220-1240),
P19(1694-1735), P24(1230-1240), P25(1482-1493), P26(384-389),
P27(2355-2389). The results of ELISA showed that P6(60% positive results)
and P19(63% positive results) testing with PT-HC of Gu An, Hebei province
were the major antigens in NS1 and in NS4 region, respectively.

For C07 try:

J Infect Dis 1996 Jan;173(1):24-31

Use of intrinsic and extrinsic helper epitopes for in vivo induction of
anti-hepatitis C virus cytotoxic T lymphocytes (CTL) with CTL epitope
peptide vaccines.

Shirai M, Chen M, Arichi T, Masaki T, Nishioka M, Newman M, Nakazawa T,
Feinstone SM, Berzofsky JA

Department of Microbiology, Yamaguchi University School of Medicine, Japan.

The induction of virus-specific cytotoxic T lymphocytes (CTL) is an
important part of vaccine strategy. CTL induction in vivo by two hepatitis C
virus (HCV) peptides containing CTL epitopes, one from the NS5 region (P17)
and one from the core (C7), was compared. P17 required covalent attachment
of a helper peptide (PCLUS3 containing a cluster of epitopes from the human
immunodeficiency virus envelope protein), whereas C7 did not. However, the
minimal decapeptide of C7, C7A10, alone did not induce CTL. The helper cells
induced by PCLUS3-17 or by C7 were shown to be CD4+ and to produce
interleukin-2 (IL-2). Thus, help can be supplied by a natural helper epitope
intrinsic to the CTL peptide, as in C7, or by attaching a helper epitope
from another protein, as in the case of P17. The cluster peptides may be
useful promiscuous helper peptides for a variety of CTL epitopes from
diverse pathogens.

Hope these were the ones you were looking for.

Regards and ciao.

karlster



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