ELISA problems

[Goran Pavlakovic]

Hi there,

I am trying to measure a fatty acid binding protein in serum.

When I do the purified standard protein dilution in water, 
ELISA is very good.  The moment I dilute my protein in serum
(immediately before the first incubation), I am not getting any 
signal. I do a classical ELISA (coating with protein of interest,
blocking, primary Ab, secondary Ab).

Any ideas on how to try to solve this?

Thanx in advance,


Goran



pavlakovic at gmx.net