CD 19 antibodies

Paul Jasper pjasper at orion.luc.edu
Tue Sep 21 16:44:19 EST 1999


Hello all,

   When you do flow cytometry or cell sorting using anti-CD19 antibodies
as a pan B cell marker ... does this antibody activate and crosslink the
CD19 molecule on the B cells??

and would this crosslinking have an effect upon mature recirculating B
cells in the absence of anti-Ig stimulation.

I'm thinking about raising an antibody against rabbit CD19 and wanted to
know if polyclonal anti-CD19 would have any effects on the B-cells as
they were sorted using this antibody... if they are all going to undergo
apoptosis using polyclonal serum, I would need to make monoclonal
antibodies and screen them for clones which do not activate CD19 ...
anyone using anti-CD19 (mouse or human) or have any thoughts? 


thanks..

Paul



-- 
___   ____   ____   ____   ____________________   ____   ____   ____   _
\  \ / \  \ / \  \ / \  \ /    Paul Jasper     \ / \  \ / \  \ / \  \ /
/\  \  /\  \  /\  \  /\   Micro/Immuno - Loyola U. /\  \  /\  \  /\  \
  \__\/  \__\/  \__\/  \__pjasper at orion.luc.edu__\/  \__\/  \__\/  \__\/



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