Cytotoxicity Assay- HELP!

Jorg Kirberg kirberg at bii.ch
Tue Sep 28 11:33:07 EST 1999


In article <37EF7918.AA7F18A8 at drexel.edu>, po at auhs.edu wrote:

> I've been working for 15 months on a simple CTL assay that doesn't seem
> to work. Here's a brief run-down on what I've been doing...
> 
> System: Chromium release
[snip]

I assume you have tested that your Chromium assay conditions are OK
by just doing some allo-specific killing (e.g. B6 -> B/c or vv 1-MLR
followed by killing P815 or EL-4s) ?
If not, I would start there since this has to work any time.

jorg



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