James F. George, Ph.D.
jgeorge at REMOVE_THISuab.edu
Mon Apr 24 08:40:06 EST 2000
We have made extensive use of the Miltenyi system with good results for both
depletion and enrichment experiments. The beads do not seem to interfere
with functional assays or the ability of the cell types we are studying to
mediate their function in vivo.
The largest drawback is, if you use their complete MACS system, it requires
a fairly hefty up-front expenditure because the magnet is expensive.
James F. George, Ph.D.
Rm 739 ZRB
Department of Surgery
University of Alabama at Birmingham
Birmingham, AL 35294-0007
jgeorge at uab.edu
"Andrew Hall" <andrew.hall at virgin.net> wrote in message
news:P21L4.755$GK5.14138 at news2-win.server.ntlworld.com...
> Slightly off thread but can I ask what you were using the Dynal beads for
> and if you know why they were not succesful. I have been using dyna-beads
> seperate out T-cells and APC populations and I usually find the cells
> aggregate by the time I have finished the procedure. From 40x10(6) PBMC I
> usually am lucky to recover 2x10(6) T-cells. If MB have a better system I
> would be interested.
> Andrew Hall
> University of Aberdeen
> andrew.hall at virgin.net
> Paula Lavery <plaver at po-box.mcgill.ca> wrote in message
> news:TPIK4.418$iK6.74789 at carnaval.risq.qc.ca...
> > Have you tried microbeads from Miltenyi Biotech? (no affiliation) They
> > much smaller than the traditional beads (ex. Dynal). We never had any
> > success with the larger beads but now routinely use the microbeads with
> > problems. Check their web site at: www.miltenyibiotec.com .
> > Good luck!
> > Paula Lavery
> > Transplant Immunology Lab.
> > Royal Victoria Hospital
> > McGill University
> > Montreal (QC) Canada
> > "If we knew what we were doing it wouldn't be called research, would
> > -- Albert Einstein
> > "Chris Weir" wrote in message
> <003a01bfa5af$75330140$0400a8c0 at cweir>...
> > Hi All,
> > I was wondering if anyone had any experience with putting
> > magnetic beads?
> > Basically I want to put a spacer between the bead and the Mab as we
> > to be getting steric
> > hinderance. If anyone could recommend some good spacers I would be
> > grateful.
> > Thanks in Advance
> > Chris Weir
> > Research/production Scientist
> > BTF Pty Ltd
> > Ph: 98892167
> > Fax: 9889 1805
> > email: chrisweir at biotechfrontiers.com
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