We routinely seperate lymphocytes (most often from murine spleen or bone
marrow) depletion of B cells, CD4, CD8 or some combination. We do both
depletions and positive selection.
I have no experience with the MACS system purifying PMNs. but have
purified PMNs from murine peritoneal exudate 4-6 hours after injection
of Casein. If I recall corectly at the 65% percoll interface we acheived
very high purity for PMNs.
I would expect that the MACS system could be used for purifying
netrophills but your concern about whether it might "effect" them is
well founded.
good luck
rjj
