detaching T cells from dishes

gaynour.sletten at vetinst.no gaynour.sletten at vetinst.no
Wed Aug 30 05:30:05 EST 2000


If you isolate and culture PBMC, T-cells don't adhere. Monocytes/macrophages
will adhere. To detach them, use magnesium- and calcium-free buffer (eg PBS)
containing 0.5mM EDTA. 


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