no pcr with stained cells

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Tue Dec 12 18:23:52 EST 2000


does adding the antibody to any PCR reaction inhibit it?
are the cell yields that problem?

Mathias Droll wrote:

> i have problems to make a RT_PCR with CD14 antibody stained Cells.
> perhabs the included sodium acid inhibits my PCR?
> without CD14 the PCR is ok.
> any ideas?
> thx






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