PCR from histology samples

The Lab Rat thelabrat at thelabrat.com
Tue Feb 1 14:30:08 EST 2000


Steve Essery wrote in message <877b5m$o95$1 at news7.svr.pol.co.uk>...
>Can anyone tell me about the feasibility of sequencing DNA from paraffin
>mounted histology samples. Any tips or protocols relating to this subject
>would be greatly appreciated.
>
>

Steve,

Try:

J Histochem Cytochem 1993 May;41(5):765-8
Rapid preparation of tissue DNA from paraffin-embedded blocks and analysis
by polymerase chain reaction.

J Virol Methods 1991 Nov;35(1):39-47
A simple method for isolation of DNA from formalin-fixed paraffin-embedded
samples for PCR.

 Diagn Mol Pathol 1999 Sep;8(3):145-51
Detection of mycobacterial nucleic acids by polymerase chain reaction in
fixed tissue specimens of patients with human immunodeficiency virus
infection. Swiss HIV Cohort Study.

Hope this helps...

JeffF

-----------------------------------------------------------------------
Jeff Fairman, Ph.D.
Senior Scientist, Pharmacogenomics Research
Clingenix, Inc.
871 Industrial Road, Suite J
San Carlos, CA 94070
(650) 598-7645 (office)
(650) 598-7641 (fax)
jfairman at clingenix.com


visit:  http://www.thelabrat.com






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