PCR from histology samples

The Lab Rat thelabrat at thelabrat.com
Tue Feb 1 14:30:08 EST 2000

Steve Essery wrote in message <877b5m$o95$1 at news7.svr.pol.co.uk>...
>Can anyone tell me about the feasibility of sequencing DNA from paraffin
>mounted histology samples. Any tips or protocols relating to this subject
>would be greatly appreciated.



J Histochem Cytochem 1993 May;41(5):765-8
Rapid preparation of tissue DNA from paraffin-embedded blocks and analysis
by polymerase chain reaction.

J Virol Methods 1991 Nov;35(1):39-47
A simple method for isolation of DNA from formalin-fixed paraffin-embedded
samples for PCR.

 Diagn Mol Pathol 1999 Sep;8(3):145-51
Detection of mycobacterial nucleic acids by polymerase chain reaction in
fixed tissue specimens of patients with human immunodeficiency virus
infection. Swiss HIV Cohort Study.

Hope this helps...


Jeff Fairman, Ph.D.
Senior Scientist, Pharmacogenomics Research
Clingenix, Inc.
871 Industrial Road, Suite J
San Carlos, CA 94070
(650) 598-7645 (office)
(650) 598-7641 (fax)
jfairman at clingenix.com

visit:  http://www.thelabrat.com

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